Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit提供了一种荧光分析方法，用于检测广泛生物靶标的细胞内钙动员情况。相对于其他商业化的染料，GFP-CERTIFIED® FLUOFORTE®染料是最亮和最灵敏的荧光钙指示剂。该试剂盒为贴壁和非贴壁细胞系提供了更少步骤，更低变异性，更简易的钙检测。此外，它既不需要洗涤步骤，也不需要添加外源性淬灭染料，避免了对受体-配体相互作用动力学的干扰。
Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit可用于细胞内钙离子的高通量检测，钙离子是重要的第二信使，常用于初步筛选中监测G蛋白偶联受体激活。
产品特色
● 可监测表达GFP细胞中的钙离子动员情况
● 最明亮、最灵敏的荧光Ca2+指示剂
● 获得的 EC50和IC50值与使用钙离子荧光探针 Rhod-4 AM和Fluo-4 AM 相当
● 更大范围的检测窗口
● 使用方便，无需洗涤，无需淬灭染料
实验示例
图2. U-2 OS细胞分别与（A）GFP-CERTIFIED® FLUOFORTE®AM染料和（B）Rhod-2 AM染料孵育。洗涤细胞并用终浓度为100nM的ATP处理，并立即成像。GFP-CERTIFIED® FLUOFORTE® AM染料比Rhod-2 AM表现出更亮的荧光强度。
产品信息
|
产品货号 |
ENZ-51020-KP010/ ENZ-51020-KP100 |
|
产品名称 |
GFP-CERTIFIED® FLUOFORTE® Calcium assay kit |
|
规 格 |
10*96wells/100*96wells |
|
短期保存 |
-20°C |
|
长期保存 |
-80°C |
|
试剂盒组分 |
Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates
High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates |
部分产品引用文献
1. Perfluorooctane sulfonate and bisphenol A induce a similar level of mast cell activation via a common signaling pathway, Fyn-Lyn-Syk activation: S.J. Park, et al.; Food Chem. Toxicol. 156, 112478 (2021)
2. horbol myristate acetate induces differentiation of THP-1 cells in a nitric oxide-dependent manner: Y.Y. Chang, et al.; Nitric Oxide 109, 33 (2021)
3. Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation: C. Trötschel, et al.; EMBO J. 39, e102723 (2020)
4. Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium: J.N. Hansen, et al.; Elife 9, e57907 (2020)
5. Interplay between sub-cellular alterations of calcium release and T-tubular defects in cardiac diseases: M. Scardigli, et al.; Front. Physiol. 9, 1474 (2018)
6. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype: H. Vienken, et al.; Sci. Rep. 7, 43575 (2017)
7. Novel insights on the relationship between T-tubular defects and contractile dysfunction in a mouse model of hypertrophic cardiomyopathy: C. Crocini, et al.; J. Mol. Cell. Cardiol. 91, 42 (2016)
8. Sulfur dioxide protects against collagen accumulation in pulmonary artery in association with downregulation of the transforming growth factor β1/smad pathway in pulmonary hypertensive rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016)
9. T-tubular electrical defects contribute to blunted β-adrenergic response in heart failure: C. Crocini, et al.; Int. J. Mol. Sci. 17, 1471 (2016)
10. Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)
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