斑马鱼作为肠道损伤模型的应用已得到充分证实，葡聚糖硫酸钠（Dextran Sulfate Sodium Salt，DSS）诱导的斑马鱼IBD模型模拟了哺乳动物IBD表型的不同特征，包括肠道中性粒细胞炎症、过多粘液生成、肠淋巴管生成增加以及前炎性细胞因子上调。
图片来源：包图网
实验动物
3dpf（days post fertilization) 斑马鱼
实验材料
DSS（Yeasen 60316ES，MW:36000~50000）
实验步骤
1. 将斑马鱼胚胎培养在含甲基蓝的E3胚胎培养基中，28.5 ℃，培养至1 dpf；
2. 1dpf 后使用不含亚甲基蓝的E3胚胎培养基，培养至3 dpf；
3. 用E3培养基配制浓度为10% DSS的储存液;
4. 用培养基稀释DSS至最大非致死剂量（DSS浓度参考：0.5%）；
5. 用0.5%的DSS处理斑马鱼，从3 dpf处理到6 dpf，观察指标[1]。
结果分析
造模成功指标：中性粒细胞数量增多、酸性粘液蛋白增多、炎性因子表达量上升。
1.嗜中性粒细胞数量：中性粒细胞数量增多，并且向肠和上皮中移动
2.酸性粘液蛋白：石蜡病理切片结合AB-PAS染色法验证，DSS浸泡后斑马鱼肠道中酸性粘液蛋白增多
3.炎性因子表达量：DSS处理后斑马鱼肠道中炎性因子表达量上升[1,2]
客户案例
图1.Yeasen DSS（Cat#60316ES）斑马鱼造模实验结果（Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557）
DSS处理组相较于对照组，酸性粘蛋白染色和粘液分泌增加，斑马鱼外周血中的嗜中性粒细胞数量增多并且向肠和上皮中移动，炎性因子表达量显著上调，肠道出现炎症反应。
翌圣DSS产品优势
翌圣提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：
1.高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建；
2.多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；
3.症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究；
4.简单易行，成模率高，重复性强；
5.联合氧化偶氮甲烷 (azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。
建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。
产品订购
|
产品名称 |
货号 |
规格 |
价格（元） |
促销价（元） |
|
Dextran Sulfate Sodium Salt（DSS） |
60316ES25 |
25 g |
1255 |
955 |
|
Dextran Sulfate Sodium Salt（DSS） |
60316ES60 |
100 g |
3855 |
2655 |
|
Dextran Sulfate Sodium Salt（DSS） |
60316ES76 |
500 g |
16855 |
9655 |
|
Dextran Sulfate Sodium Salt（DSS） |
60316ES80 |
1 kg |
32855 |
18655 |
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产品已发表文章(部分)
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2022
[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143
[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451
[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076
[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869
2021
[1] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
[2] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717
[3] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943
[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
2020
[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
BEFORE 2020
[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973
[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048
[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868
[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943
相关专题
1.不同分子量葡聚糖硫酸钠选购指南：高品质DSS葡聚糖硫酸钠盐
2.DSS结肠炎造模整体解决方案：Dextran Sulfate Sodium Salt（DSS） 溃疡性结肠炎模型的建立 (yeasen.com)
参考文献：[1] Oehlers SH, et al. Dis Model Mech. 2012 Jul;5(4):457-67.[2] Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557.