当对小鼠样本进行 IHC 实验时，CST 更推荐您使用兔单克隆抗体而不是鼠单克隆抗体，为什么，这么做有什么好处？3 步让您了解原因！
使用小鼠单克隆抗体会造成小鼠组织结果无法解读
如经适当验证，小鼠单克隆抗体会特异性地对目的靶标染色，但数据解读很可能因使用抗小鼠 IgG 检测试剂（二抗）同时能检测内源性小鼠 IgG 而受到影响。如图1，依次使用一种 α-平滑肌肌动蛋白（α-SMA）小鼠单克隆抗体和一种抗小鼠 IgG 检测试剂对石蜡包埋的小鼠肺细胞进行免疫组织化学 (IHC) 分析，结果显示出预期的特异性染色，但由于存在抗小鼠 IgG 二抗，还显示出背景染色过度。避免小鼠 IgG 背景的唯一办法是在你的实验步骤中添加费力的步骤，或换用一种非啮齿类抗体，例如联合使用兔单克隆抗体和抗兔 IgG 的二抗。
图1 ，注：小鼠 IgG 二抗导致背景染色过度。
抗小鼠 IgG 二抗会造成小鼠抗小鼠背景染色
使用小鼠一抗需要抗小鼠 IgG 二抗或检测试剂，这同时会对一抗和小鼠组织样品的 IgG 蛋白进行染色。为了让您直观地观察，图2展示的是在没有一抗的情况下，使用 SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 对石蜡包埋的小鼠肺细胞进行 IHC 分析。
兔单克隆抗体无需额外的实验步骤即可产生可解读的小鼠组织结果
使用在小鼠组织中经 IHC 验证的兔单克隆一抗可克服小鼠抗小鼠染色，并发现明确且可解读的结果。图3 所示的是使用 α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb #19245 对石蜡包埋的小鼠肺细胞进行的 IHC 分析。
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产品名称 |
货号 |
应用 |
反应性 |
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细胞生物学 |
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Bax (D3R2M) Rabbit mAb (Rodent Preferred) |
WB、IP、IHC-P |
M、R |
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N-Cadherin (D4R1H) XP® Rabbit mAb |
WB、IP、IHC-P、IF-IC |
H、M |
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CD44 (E7K2Y) XP® Rabbit mAb |
WB、IHC-P |
H、M、R |
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EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) |
WB、IP、IHC-P、 IF-F、IF-IC、F |
M |
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EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) |
WB、IP、IHC-P |
H、M、R |
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HER3/ErbB3 (D22C5) XP® Rabbit mAb |
WB、IP、IHC-P、 IF-IC、F |
H、M、(R) |
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Integrin ß3 (D7X3P) XP® Rabbit mAb |
WB、IHC-P |
H、M |
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Keratin 5 (E2T4B) XP® Rabbit mAb (IHC Preferred) |
IHC-P |
H、M |
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Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) |
IHC-P |
M |
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Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P、IF-F |
M |
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Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P、 IF-IC、F |
M、R |
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PCNA (D3H8P) XP® Rabbit mAb |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R、Mk |
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Perforin (E3W4I) Rabbit mAb |
WB、IHC-P |
M |
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α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb |
WB、IP、IHC-P、IF-F |
H、M、R |
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Vinculin (E1E9V) XP® Rabbit mAb |
WB、IHC-P、F |
H、M、R、Mk |
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发育生物学 |
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Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R、Mk、 Z、Dg |
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HES1 (D6P2U) Rabbit mAb |
WB、IP、IHC-P |
H、M、R、Mk |
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SMAD4 (D3R4N) XP® Rabbit mAb |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Mk |
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Sox9 (D8G8H) Rabbit mAb |
WB、IHC-P、IF-IC、F |
H、M、(M、R) |
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YAP (D8H1X) XP® Rabbit mAb |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Hm、 Mk、(B、Hr) |
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表观遗传学 |
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ARID1A/BAF250A (D2A8U) Rabbit mAb |
WB、IHC-P、ChIP |
H、M、R、Mk |
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EED (E4L6E) XP® Rabbit mAb |
WB、IP、IHC-P、IF-IC、 F、ChIP |
H、M、R、Mk |
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Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Mk |
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代谢 |
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Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb |
WB、IP、IHC-P |
H、M、R、C、Z、 B、Pg) |
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Arginase-1 (D4E3MTM ) XP® Rabbit mAb |
WB、IHC-P、IF-F、 IF-IC、F |
H、M、R |
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COX IV (D6I4K) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P |
M、R |
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SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P、IF-IC |
M、R |
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神经科学 |
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Adora2a/Adenosine Receptor A2a (E5I8X) XP® Rabbit mAb (Rodent Specific) |
IHC-P、IF-F |
M、R |
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GFAP (E4L7M) XP® Rabbit mAb |
WB、IHC-P、IF-F |
H、M、R |
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Myelin Basic Protein (D8X4Q) XP® Rabbit mAb |
WB、IHC-P、IF-F、IF-IC |
H、M、R |
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NCAM1 (CD56) (E7X9M) XP® Rabbit mAb |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R |
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NeuN (D4G4O) XP® Rabbit mAb |
WB、IHC-P、IF-F |
H、M、R |
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Tau (D1M9X) XP® Rabbit mAb |
WB、IHC-P、IF-F、IF-IC |
H、M、R |
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免疫学 |
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Btk (D3H5) Rabbit mAb |
WB、IP、IHC-P、F |
H、M、(R、Hm、B、Dg、Pg、Hr) |
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CD3ε (D4V8L) Rabbit mAb |
WB、IHC-P |
M |
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CD4 (D7D2Z) Rabbit mAb |
WB、IHC-P |
M、R |
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CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) |
WB、IHC-P |
M |
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CD11c (D1V9Y) Rabbit mAb |
WB、IHC-P、IF-F、IF-IC |
M |
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CD19 (D4V4B) XP® Rabbit mAb |
WB、IP、IHC-P |
H、M、(B) |
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CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb |
WB、IHC-P |
M |
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CD39/NTPDase 1 (E2X6B) XP® Rabbit mAb (Mouse Specific) |
WB、IHC-P |
M |
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CD40 (E2Z7J) Rabbit mAb |
WB、IP、IHC-P |
M |
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CD45 (D3F8Q) Rabbit mAb |
P、IHC-P、IF-F、IF-IC |
M |
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F4/80 (D2S9R) XP® Rabbit mAb |
WB、IP、IHC-P |
M |
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Granzyme B (E5V2L) Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P、F |
M |
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FoxP3 (D6O8R) Rabbit mAb |
IHC-P、IF-F、F |
M |
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FcγRIIB (D8F9C) XP® Rabbit mAb（Mouse Specific) |
WB、IP、IHC-P、F |
M |
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NT5E/CD73 (D7F9A) Rabbit mAb |
WB、IHC-P |
H、M、R |
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PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P、IF-F、IF-IC、F |
M、(R、Hm) |
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PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) |
IHC-P |
M |
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PD-L2 (D6L5A) Rabbit mAb |
IHC-P |
M |
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S100A9 (D3U8M) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P、 IF-F、F |
M、R |
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STING (D2P2F) Rabbit mAb |
WB、IP、IHC-P |
H、M |
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TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P |
M |
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VCAM-1 (D2T4N) Rabbit mAb (Mouse Specific) |
WB、IHC-P |
M |
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