内皮细胞分化鞘脂G蛋白偶联受体1抗体-分析方法-资讯-生物在线

内皮细胞分化鞘脂G蛋白偶联受体1抗体

作者:上海雅吉生物科技有限公司 2020-11-05T00:00 (访问量:853)

  

中文名称 内皮细胞分化鞘脂G蛋白偶联受体1抗体
别    名 CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; G protein coupled sphingolipid receptor; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1; S1PR1_HUMAN; Sphingosine 1 phosphate receptor Edg 1; Sphingosine 1 phosphate receptor EDG1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1phosphate receptor type 1 S1P1.  

 

研究领域 细胞生物  信号转导  细胞凋亡  细胞粘附分子  G蛋白偶联受体  内皮细胞  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Horse, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 44kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human EDG1/CD363:51-150/382 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking.

Function:
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.

Post-translational modifications:
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P21453

Gene ID:
1901

Database links:

Entrez Gene: 281135 Cow

Entrez Gene: 100050049 Horse

Entrez Gene: 1901 Human

Entrez Gene: 13609 Mouse

Entrez Gene: 29733 Rat

Omim: 601974 Human

SwissProt: Q5E9P3 Cow

SwissProt: P21453 Human

SwissProt: O08530 Mouse

SwissProt: P48303 Rat

Unigene: 154210 Human

Unigene: 982 Mouse

Unigene: 109455 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

研究发现,S1P1与VEGF肩并肩相互协作,从而促进血管生长。VEGF是很多不同抗癌药物的作用靶标。血管新生在很多疾病是异常的。靶向S1P1和VEGF可能要比只使用VEGF抑制剂更加有效地治疗疾病;S1P1是一种关键性的血管新生反应性基因。研究人员证实当新的血管网络形成时,由此产生的血液流动激活血管内皮细胞表面上的S1P1,并把信号传递到这些细胞内部从而让新形成的血管网络稳定化。因此阻断S1P1可能有助于切断给癌性肿瘤提供资源的血液供应。
产品图片 Sample:
H9C2 Cell (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-EDG1 (bs-7112R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 50 kD
Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti-EDG1 (bs-7112R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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