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Ephrin Type-B Receptor 4 ( EphB4 / EPHB4 / HTK ) Antibody Datasheet
 
 
 
EphB4 / EPHB4 / HTK抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human EphB4 protein ( Catalog#10235-H08H  )
 
 
 
 
 
克隆号 :
 
 
 
11F11G1B1
 
 
 
 
Ig Type :
 
 
Mouse  IgG1
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human EphB4 / HTK extracellular domain ( rhEphB4; Catalog#10235-H08H; NP_004435.3; Met 1-Ala 539 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
EphB4 / EPHB4 / HTK抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human EphB4 / HTK
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Western blot :
 
This antibody can be used at 1 - 2 ug/mL with the appropriate secondary reagents to detect EphB4 in WB. Using a DAB detection system, the detection limit for EphB4 is approximately 20 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
Image : 
 
 
Western blot  –  EphB4 / HTK antibody 
 
 
The detection limit for human EphB4 is approximately 4ng/lane under reducing conditions
 
 
The detection limit for human EphB4 is approximately 20ng/lane under non-reducing conditions
 
 
 
 
 
EphB4 / EPHB4 / HTK Antibody Related Products &Topics
 
 
EphB4 Antibody 相关研究领域:
 
Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>EphB4
 
Neuroscience>>Axon Guidance>>Ephrin & Eph Receptor>>EphB4
 
Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Hemangioblast Marker>>EphB4
 
Cancer>>Growth Factor & Receptor>>Ephrin & Eph Receptor>>EphB4
 
Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>EphB4
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
EphB4
 
Human
 
EphB4/Fc Protein, Recombinant
 
10235-H02H
 
 
 
EphB4
 
Human
 
EphB4 Protein, Recombinant
 
10235-H08H
 
 
 
EphB4
 
Human
 
EphB4 Protein, Recombinant
 
10235-HCCH
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human 
 EphB4
 
WB
 
Mouse Monoclonal Antibody
 
10235-MM03
 
 
 
Human 
 EphB4
 
ELISA
 
Mouse Monoclonal Antibody
 
10235-MM04
 
 
 
Human 
 EphB4
 
WB, ELISA
 
Rabbit Monoclonal Antibody
 
10235-RM02
 
 
 
Human 
 EphB4
 
ELISA
 
Rabbit Polyclonal Antibody
 
10235-RP01
 
 
 
Human 
 EphB4
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10235-RP05
 
 
 
 
 
EphB4 / EPHB4 / HTK Antibody Background
 
 
EphB4, also known as Htk, Myk-1, is a member of the Eph family of receptor tyrosine kinases (RTK). The Ephs are divided into two classes based on their extracellular structure and ligand specificity, and the transmembrane receptor binding preferentially to ephrin-B ligands is referred to as EphB. Sharing common structure organization, all Eph receptors contain an N-terminal Ig-like domain, a cysteine-rich region and two fibronectin type III domains within the ECD, as well as the typical cytoplasmic tyrosine kinase domain. EphB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EphB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis, and tumor growth. In contrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EphB4 is associated with prostate cancer and highly malignant breast cancers, and accordingly, EphB4 has potential applications as a therapeutic candidate.
 
 
参考文献
 
 
1.   Bennett, BD. et al., 1994, J. Biol. Chem. 269: 14211-8.
 
2.   Noren, NK. et al., 2004, Proc. Natl. Acad. Sci. 101: 5583-8.
 
3.   Murai, KK. et al., 2003, J. Cell. Sci. 116: 2823-32.
 
4.   Noren, NK. et al., 2006, Nat. Cell. Biol. 8: 815-25.
 
5.   Xia, G. et al., 2005, Cancer Res. 65: 4623-32.
 
6.   Kertesz, N. et al., 2006, Blood. 107: 2330-38.
 
 
 
 
 
 
 
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